| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.138235 |
| Chromosome: | chromosome 2 |
| Location: | 3638066 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095145 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGTGGAGGGCGTGGCACGGGGCGGACAG |
| Internal bar code: | CGTTTCTGAGCACCCCGAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 874 |
| LEAP-Seq percent confirming: | 94.0299 |
| LEAP-Seq n confirming: | 134899 |
| LEAP-Seq n nonconfirming: | 8565 |
| LEAP-Seq n unique pos: | 135 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGAGGACTCCTTTTGTGC |
| Suggested primer 2: | GTCGTCCTGCCGTGATTATT |