| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.138259 |
| Chromosome: | chromosome 16 |
| Location: | 3581662 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g682013 | (1 of 2) PTHR30545:SF2 - SUGAR FERMENTATION STIMULATION PROTEIN A | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCCCTTCACAGCCGCCTCCGCCTTCCCT |
| Internal bar code: | GTCCTCCCGAATGTCTTCTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 681 |
| LEAP-Seq percent confirming: | 99.6445 |
| LEAP-Seq n confirming: | 17939 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGGTGAGGTGTGGTGTCA |
| Suggested primer 2: | GGACGCTAGAAAGCAGGATG |