| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.138270 |
| Chromosome: | chromosome 3 |
| Location: | 7581089 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g204752 | (1 of 1) K00565 - mRNA (guanine-N7-)-methyltransferase (RNMT) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCCGCTGCCGCTTGGGCGGCCCGCCGCC |
| Internal bar code: | ATTGGGGCGGGAACACCACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 81 |
| LEAP-Seq percent confirming: | 70.6798 |
| LEAP-Seq n confirming: | 863 |
| LEAP-Seq n nonconfirming: | 358 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAGCAACAGGCAGTAGGC |
| Suggested primer 2: | GGAGGGGAGGGTTGTTACAT |