| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.138273 |
| Chromosome: | chromosome 3 |
| Location: | 6076793 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g191500 | (1 of 2) PTHR11373:SF4 - DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE SAMHD1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGTGCCAGCGGCGTTAGCACAGGGGTTA |
| Internal bar code: | GAGCTCGGTTTTTCCAGCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 564 |
| LEAP-Seq percent confirming: | 98.8773 |
| LEAP-Seq n confirming: | 1233 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGTCTATGACGCCCTTGC |
| Suggested primer 2: | CTGTGTTTTGTGGGATGGTG |