| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.138284 |
| Chromosome: | chromosome 16 |
| Location: | 6469656 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g673200 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCTAGACGAGAGGGCTGTGGCACTTGGT |
| Internal bar code: | CTGCGGGAGGCGGCCTCCCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1211 |
| LEAP-Seq percent confirming: | 97.5565 |
| LEAP-Seq n confirming: | 2116 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGCCTGAAGGTGAGGTC |
| Suggested primer 2: | CATGACCCCAAACGTAATCC |