| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.138315 |
| Chromosome: | chromosome_7 |
| Location: | 3707487 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g337650 | PDC1 | Mitochondrial pyruvate dehydrogenase complex, E1 component, alph | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAAGAGAAGATTAGGGGGAGGTGGTGGTGG |
| Internal bar code: | CAGTGTGTGACCGAATTCCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 220 |
| LEAP-Seq percent confirming: | 48.538 |
| LEAP-Seq n confirming: | 498 |
| LEAP-Seq n nonconfirming: | 528 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCTTCTGTCGCTGTCTC |
| Suggested primer 2: | CGACAAGGAGGTCAAGAAGG |