| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.138341 |
| Chromosome: | chromosome_2 |
| Location: | 603791 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g077800 | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTTCAAAGTCGCCCCCTCGTGCTGAACTAT |
| Internal bar code: | AGCATGGTCAGTGCGGCTGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 440 |
| LEAP-Seq percent confirming: | 51.714 |
| LEAP-Seq n confirming: | 528 |
| LEAP-Seq n nonconfirming: | 493 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGTGTACAGAGGAATGGT |
| Suggested primer 2: | TCTCCAGAAAGGAAACCCCT |