Insertion junction: LMJ.RY0402.138345_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.138345
Chromosome:chromosome 1
Location:2439925
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g013650 (1 of 3) PF15612 - WSTF, HB1, Itc1p, MBD9 motif 1 (WHIM1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGAGCGTACTCGAGGGTTTGCCATCGACC
Internal bar code:TGCTCTTGATTGCAACACCTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:728
LEAP-Seq percent confirming:99.7
LEAP-Seq n confirming:7975
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CGCACCAACTACTCAAAGCA
Suggested primer 2:ACGGTTATGCCCAGTGAGAC