| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.138346 |
| Chromosome: | chromosome 2 |
| Location: | 2208589 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g089750 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGAGGCAGCACCAACAGCACCAACAGCA |
| Internal bar code: | CTTGTCGGGTTCGTATAGTACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 21 |
| LEAP-Seq percent confirming: | 91.1882 |
| LEAP-Seq n confirming: | 1604 |
| LEAP-Seq n nonconfirming: | 155 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACACATCATGGCTCTCG |
| Suggested primer 2: | AGCAACAGCAACAGCAACAG |