| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.138346 |
| Chromosome: | chromosome 6 |
| Location: | 2270125 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g266800 | (1 of 3) PTHR30535//PTHR30535:SF2 - VITAMIN B12-BINDING PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTTGACGCGGTCAAGCTGTTGGTGGGCC |
| Internal bar code: | AGCCCCAGCCGCGTAACTTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 515 |
| LEAP-Seq percent confirming: | 88.2353 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGGGATTAGAGGGACTGG |
| Suggested primer 2: | GGCAACAGCTTAACGTGACA |