| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.138353 |
| Chromosome: | chromosome 16 |
| Location: | 2321681 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g659450 | (1 of 3) 2.4.1.228 - Lactosylceramide 4-alpha-galactosyltransferase / Histo-blood group P(k) UDP-galactose | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTTGCTACCCGCACGGTGCAGGCTGGCA |
| Internal bar code: | GCGAAGGCGGGTTACACTCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1302 |
| LEAP-Seq percent confirming: | 99.4989 |
| LEAP-Seq n confirming: | 65526 |
| LEAP-Seq n nonconfirming: | 330 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAGAACCTGTCTGCTGGG |
| Suggested primer 2: | GATACACGCCAGGGTCAACT |