| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.138355 |
| Chromosome: | chromosome 6 |
| Location: | 3512483 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278107 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTTTGAGTAGTTAATCCATCCACCGCTC |
| Internal bar code: | TACTAAGACCTAACCTGTGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 419 |
| LEAP-Seq percent confirming: | 97.7289 |
| LEAP-Seq n confirming: | 5336 |
| LEAP-Seq n nonconfirming: | 124 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGAGTCCATTTGTTGGC |
| Suggested primer 2: | TTGATAGAAGCGTTCGGGAC |