| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.138355 |
| Chromosome: | chromosome 9 |
| Location: | 6638512 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g408650 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGAGAAGCGCGCACACTTTTTTACGCCC |
| Internal bar code: | CGCGCGGTCAATCGGGTTAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 509 |
| LEAP-Seq percent confirming: | 98.7768 |
| LEAP-Seq n confirming: | 323 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGAACACTGGGAACACTG |
| Suggested primer 2: | ACTAGTGCCACCGCAAAAAC |