| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.138396 |
| Chromosome: | chromosome 16 |
| Location: | 5638145 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679500 | NUOB8 | (1 of 1) K03946 - NADH dehydrogenase (ubiquinone) 1 alpha subcomplex subunit 2 (NDUFA2); NADH:ubiquinone oxidoreductase 11 kDa subunit | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAGCACAGCAAGGCGCCACACCGCAGAT |
| Internal bar code: | ACCTCGGTAGGCTGTTGGGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 691 |
| LEAP-Seq percent confirming: | 98.2908 |
| LEAP-Seq n confirming: | 3853 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGGTGTGCATTTGTTGAC |
| Suggested primer 2: | AGTCTATGCCCAAGTGGGTG |