| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.138419 |
| Chromosome: | chromosome 17 |
| Location: | 3084712 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g720950 | SRD3 | 3-oxo-5-alpha-steroid 4-dehydrogenase family protein; (1 of 1) 1.3.1.94 - Polyprenol reductase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCGGTCAGGTCCCACATGGCCTCCGCAC |
| Internal bar code: | CCGTGTCTGGGTGGAGAGGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 910 |
| LEAP-Seq percent confirming: | 99.2152 |
| LEAP-Seq n confirming: | 4551 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATCAAGTGCAAGGAATCGG |
| Suggested primer 2: | TTCCGAACATTTTGGAGAGG |