| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.138431 |
| Chromosome: | chromosome 8 |
| Location: | 1126901 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g362600 | BLA1 | (1 of 2) PF00144 - Beta-lactamase (Beta-lactamase); beta-lactamase family protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATTACGGTGCGCGTGTGGGTACGTACCGT |
| Internal bar code: | TACAATTCCCTAGACCGCGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1074 |
| LEAP-Seq percent confirming: | 99.5544 |
| LEAP-Seq n confirming: | 6703 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCAACCCTATACACTCGCC |
| Suggested primer 2: | GTTTATGCATGCGTGTGACC |