| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.138444 |
| Chromosome: | chromosome_5 |
| Location: | 2202062 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g234652 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCACCGCGCTTTGCTCATTCGCGGCCGCT |
| Internal bar code: | CAACGTTTACATAGCCTCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 289 |
| LEAP-Seq percent confirming: | 89.1757 |
| LEAP-Seq n confirming: | 4457 |
| LEAP-Seq n nonconfirming: | 541 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATGTTTGCTGTTGGGGCT |
| Suggested primer 2: | TGTTACATACCAGGCGGTCA |