| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.138452 |
| Chromosome: | chromosome 2 |
| Location: | 2225712 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g089900 | SCM1,SCAMP5 | (1 of 1) PTHR10687 - SECRETORY CARRIER-ASSOCIATED MEMBRANE PROTEIN SCAMP; secretory carrier membrane protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTTTACAATAGTCGCCCCTGCCGCAGTA |
| Internal bar code: | GTGTGGAGATTTCTCGCGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 708 |
| LEAP-Seq percent confirming: | 98.6321 |
| LEAP-Seq n confirming: | 8436 |
| LEAP-Seq n nonconfirming: | 117 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGATGCATAGCAGAGCGTC |
| Suggested primer 2: | GAGTGCGGAGAAGGTTGAAG |