| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.138485 |
| Chromosome: | chromosome 13 |
| Location: | 2079404 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g577400 | ELG16 | (1 of 1) IPR000742//IPR004263//IPR009030//IPR013111 - EGF-like domain // Exostosin-like // Insulin-like growth factor binding protein, N-terminal // EGF-like domain, extracellular; Exostosin-like glycosyltransferase 16 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGCTCTCTCTGGGCGGGGCCGATGGCAA |
| Internal bar code: | GATCTCCACGCCAAACGTATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 170 |
| LEAP-Seq percent confirming: | 99.7553 |
| LEAP-Seq n confirming: | 1631 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGCTCCATTTACTCCATCC |
| Suggested primer 2: | ACAACACACACGAGCAAAGC |