| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.138495 |
| Chromosome: | chromosome 6 |
| Location: | 3652740 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278132 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATAACACTTCGTGGTCTGTTCAGCTTCG |
| Internal bar code: | TGTGGGCAGAGTAAACCCATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1166 |
| LEAP-Seq percent confirming: | 98.6762 |
| LEAP-Seq n confirming: | 6187 |
| LEAP-Seq n nonconfirming: | 83 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCAAGCTTAGATTTGGCT |
| Suggested primer 2: | AGGGACTTGGCCTAAGCAAT |