| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.138508 |
| Chromosome: | chromosome 7 |
| Location: | 184832 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g313450 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGACGTGCCAGGTAGCCCCCGAACACGG |
| Internal bar code: | AACCCCGTTCGAGTGCAAGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 277 |
| LEAP-Seq percent confirming: | 51.5027 |
| LEAP-Seq n confirming: | 1885 |
| LEAP-Seq n nonconfirming: | 1775 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCATGGAATATGGTATCG |
| Suggested primer 2: | GGCTGATGTGTTGGTGACAG |