Insertion junction: LMJ.RY0402.138514_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.138514
Chromosome:chromosome 1
Location:5827832
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g041583 (1 of 2) PF03366 - YEATS family (YEATS) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAAGACCCGCCAGACTGTTGCTTCCATC
Internal bar code:TGCGAGTCCGCGGTTATGGAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:740
LEAP-Seq percent confirming:98.4691
LEAP-Seq n confirming:8169
LEAP-Seq n nonconfirming:127
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:CTGACAGGGCTACTGCTGGT
Suggested primer 2:GCTGTGGCGACTCTAGGAAC