| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.138527 |
| Chromosome: | chromosome_11 |
| Location: | 2533659 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g476050 | ODA2,DHC15,DYHG | Flagellar outer arm dynein heavy chain gamma | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TAAGAATGTATCGTATGTGCAGGATGTCAA |
| Internal bar code: | TGCGGCGGTTTAGCTCCCCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 875 |
| LEAP-Seq percent confirming: | 96.5131 |
| LEAP-Seq n confirming: | 4650 |
| LEAP-Seq n nonconfirming: | 168 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGGTGAGTTTGGGTTCCT |
| Suggested primer 2: | AGGAATTTTGATGGTGGCTG |