| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.138536 |
| Chromosome: | chromosome 17 |
| Location: | 605575 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g700133 | WNK1 | (1 of 5) K08867 - WNK lysine deficient protein kinase [EC:2.7.11.1] (WNK, PRKWNK); WNK protein kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGTCACGTACATCACCGCTGGATTGAGT |
| Internal bar code: | TAGGTCTAAAGTGTATGGGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 744 |
| LEAP-Seq percent confirming: | 99.6258 |
| LEAP-Seq n confirming: | 2929 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTTACGGGTGGTGTCATA |
| Suggested primer 2: | TTGAACAGCAGGAAGCAATG |