Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.138560 |
Chromosome: | chromosome_11 |
Location: | 1698531 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre11.g467781 | FAP151 | ABC transporter-like and Flagellar Associated Protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACTTTTCTGACACGCTCCTCCTGAAGACAT |
Internal bar code: | GGGCGGCCCTCCGTCGACCCGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 811 |
LEAP-Seq percent confirming: | 90.683 |
LEAP-Seq n confirming: | 2735 |
LEAP-Seq n nonconfirming: | 281 |
LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTCGTACTATTGCCGCACCT |
Suggested primer 2: | ATGGGAAGGAAGGAAGGAGA |