| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.138593 |
| Chromosome: | chromosome 1 |
| Location: | 1440819 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g007251 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTAAAGTCAGTCAGGTCAAAGTTTCGCA |
| Internal bar code: | CGGATCCGCTCGGCGCAGCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 839 |
| LEAP-Seq percent confirming: | 99.1296 |
| LEAP-Seq n confirming: | 2961 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGATTGGGTGCTTGTTGTA |
| Suggested primer 2: | TTCCCCCTTCTGTCGTATTG |