| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.138595 |
| Chromosome: | chromosome 3 |
| Location: | 8715609 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g204577 | DNJ31 | (1 of 1) IPR000104//IPR001623//IPR003072 - Antifreeze protein, type I // DnaJ domain // Orphan nuclear receptor, NOR1 type; DnaJ-like protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCTCGCAACAGCCGCCGCAGTACGGCTT |
| Internal bar code: | AATAATTTTTTTTAGCTCTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 133 |
| LEAP-Seq percent confirming: | 66.3793 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCGGAGGTGTAGGTCAAC |
| Suggested primer 2: | TTAAATGACTTCGCACGCTG |