| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.138600 |
| Chromosome: | chromosome 9 |
| Location: | 6516263 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g407550 | (1 of 1) IPR000104//IPR003034 - Antifreeze protein, type I // SAP domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCTGGCGGGGCTGCTGGGTTTGGCAGTA |
| Internal bar code: | ATGCCGAGATGTCGGGCCCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 135 |
| LEAP-Seq percent confirming: | 71.2905 |
| LEAP-Seq n confirming: | 6491 |
| LEAP-Seq n nonconfirming: | 2614 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGTCCATGATATCCCAAA |
| Suggested primer 2: | CTGGAGTTGATCCCACTCGT |