| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.138602 |
| Chromosome: | chromosome 6 |
| Location: | 8212643 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g305600 | ADH9 | Alcohol dehydrogenase; (1 of 1) 1.1.1.329 - 2-deoxy-scyllo-inosamine dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTGCAGCAGTGTGGTGCCGGTGAAGAGT |
| Internal bar code: | ACAGCTTACTGTTTGTAACATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1288 |
| LEAP-Seq percent confirming: | 99.049 |
| LEAP-Seq n confirming: | 8749 |
| LEAP-Seq n nonconfirming: | 84 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATGACCTCGCACTCTCAC |
| Suggested primer 2: | CAGCAAGGGAACTCTCAAGG |