| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.138632 |
| Chromosome: | chromosome_16 |
| Location: | 3101943 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g665450 | FAP48,RYR1 | Ryanodine-inositol 1,4,5-triphosphate receptor Ca2+ channel | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTTAGCTACCGTATCCCCCGAGTGTTCTCC |
| Internal bar code: | ATCAAGTCTATGAATAACTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 714 |
| LEAP-Seq percent confirming: | 99.8285 |
| LEAP-Seq n confirming: | 1164 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCAGCTCAATGTCGTCAA |
| Suggested primer 2: | ATGTAACGACGCCAAGGTTC |