Insertion junction: LMJ.RY0402.138638_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.138638
Chromosome:chromosome 3
Location:7968469
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g201950 (1 of 1) IPR003882//IPR008752//IPR024079 - Pistil-specific extensin-like protein // Peptidase M11, gametolysin // Metallopeptidase, catalytic domain 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAGTCGTTCTGTCAATCGCTATTATGCTG
Internal bar code:GGGGCCACAACCCTACTGCAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:53
LEAP-Seq percent confirming:81.1321
LEAP-Seq n confirming:43
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:ATGTTTAGGGCTGGTGTTGC
Suggested primer 2:TCATCCGTACTGCTGCTGTC