| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.138649 |
| Chromosome: | chromosome 3 |
| Location: | 296325 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144584 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTACCCGGCCCAGGGCACGCGCCTCTGA |
| Internal bar code: | TCGTCCGACGAAGTGTACACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 981 |
| LEAP-Seq percent confirming: | 99.8548 |
| LEAP-Seq n confirming: | 8250 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGCCTTTGGAAGTTTGCT |
| Suggested primer 2: | ACAATGCGGAGGGTGTCTAC |