| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.138659 |
| Chromosome: | chromosome 9 |
| Location: | 4620842 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396587 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGTGGAACACGCCCTGGGGCATCTGGAT |
| Internal bar code: | ACGTCGACTGTCGGACCGACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 808 |
| LEAP-Seq percent confirming: | 98.4745 |
| LEAP-Seq n confirming: | 3744 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTCCCTTTGCTTCACGAC |
| Suggested primer 2: | GGCAGTAGTGTGTGTGGTGG |