| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.138666 |
| Chromosome: | chromosome_3 |
| Location: | 5432355 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g185050 | FAP53 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCAAGCAGATGGAGATCTCGGAGGCCGAG |
| Internal bar code: | AGCTACACGTGAAGCCGACTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 808 |
| LEAP-Seq percent confirming: | 98.9378 |
| LEAP-Seq n confirming: | 1304 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACATGCTCACCAATCGAAC |
| Suggested primer 2: | GAGAAGGCGTTCATCCAGAG |