| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.138669 |
| Chromosome: | chromosome 6 |
| Location: | 8480268 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g307650 | (1 of 1) PF12138 - Spherulation-specific family 4 (Spherulin4) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGGGCGCAGGTGATGATCCCTCGCATG |
| Internal bar code: | ATTTAATATCGGAGAGTTCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 694 |
| LEAP-Seq percent confirming: | 99.5213 |
| LEAP-Seq n confirming: | 1871 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAAGGCAGCTCAAGGATGC |
| Suggested primer 2: | GGATGCGTGACAGTAGGGAT |