| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.138672 |
| Chromosome: | chromosome_16 |
| Location: | 1429487 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g652400 | FAL18 | Similar to Flagellar Associated Protein FAP183 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCCCCGCCGACACCCTACCGCAGCTGTACT |
| Internal bar code: | TACTGACTTGCCCGGCCAAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 867 |
| LEAP-Seq percent confirming: | 98.9056 |
| LEAP-Seq n confirming: | 6959 |
| LEAP-Seq n nonconfirming: | 77 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGGAGCAAGAAAAACGC |
| Suggested primer 2: | GGCTAAAAGCACGTCCTCAG |