| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.138673 |
| Chromosome: | chromosome 17 |
| Location: | 3902905 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g728350 | (1 of 21) PF02469 - Fasciclin domain (Fasciclin) | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGCGAGGCGAATGCGGGTTGGCGCGGTC |
| Internal bar code: | AACCTGCGCTGTCCCCGACGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1045 |
| LEAP-Seq percent confirming: | 99.2736 |
| LEAP-Seq n confirming: | 820 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGTACGCTTTGCTCCTCC |
| Suggested primer 2: | TGAGCAAGTGAATGAAAGCG |