Insertion junction: LMJ.RY0402.138697_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.138697
Chromosome:chromosome 8
Location:4224036
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre08.g380550 SDR14 (1 of 2) IPR002347//IPR013968 - Glucose/ribitol dehydrogenase // Polyketide synthase, ketoreductase domain; Short-chain dehydrogenase/reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGTTGCAGATACCAGCCCAAACTAAAGC
Internal bar code:GCAAACTACGTTGAATAGCGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:234
LEAP-Seq percent confirming:84.671
LEAP-Seq n confirming:2342
LEAP-Seq n nonconfirming:424
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:ATAACCAAATGGCGAACGAC
Suggested primer 2:GTGACCTTCACAGATGGCCT