Insertion junction: LMJ.RY0402.138724_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.138724
Chromosome:chromosome 1
Location:2933278
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g017900 (1 of 8) IPR011047//IPR018391 - Quinonprotein alcohol dehydrogenase-like superfamily // Pyrrolo-quinoline quinone beta-propeller repeat 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAGACAGTAAATAGTGAAGTCGGAGCTGG
Internal bar code:CCGACCAGGGGATGAGGCCATC

Confirmation - LEAP-Seq

LEAP-Seq distance:1047
LEAP-Seq percent confirming:99.655
LEAP-Seq n confirming:10977
LEAP-Seq n nonconfirming:38
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:TAGCTCGCAACTGGATCCTT
Suggested primer 2:CAGTAAGGCAGCTAGGGCAC