| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.138741 |
| Chromosome: | chromosome 1 |
| Location: | 4416168 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g029900 | (1 of 3) 3.6.3.17 - Monosaccharide-transporting ATPase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCAGGGAGGGGGTACACAAGGCAGCAGT |
| Internal bar code: | CGGGCGACAGACGTTTCGGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 675 |
| LEAP-Seq percent confirming: | 99.7888 |
| LEAP-Seq n confirming: | 1890 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGGAACCATCTGCCTGAG |
| Suggested primer 2: | CGTATGTCCGGTCTTCAGGT |