| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.138744 |
| Chromosome: | chromosome 16 |
| Location: | 1179524 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g650625 | (1 of 1) PTHR11757:SF3 - OLIGOPEPTIDASE B | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGACCTGAGGCTCGCAAAACACCTCACA |
| Internal bar code: | GAGGCCGCGCGCGCTGCCGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 676 |
| LEAP-Seq percent confirming: | 99.232 |
| LEAP-Seq n confirming: | 9691 |
| LEAP-Seq n nonconfirming: | 75 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCAATAGCAGGAAGCGAG |
| Suggested primer 2: | CTACATCCGCTCCTACTCGC |