Insertion junction: LMJ.RY0402.138746_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.138746
Chromosome:chromosome 7
Location:1585612
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g324800 SNE6 (1 of 8) PTHR10366//PTHR10366:SF411 - NAD DEPENDENT EPIMERASE/DEHYDRATASE // HIGH CHLOROPHYLL FLUORESCENCE PHENOTYPE 173 PROTEIN; Cinnamoyl-CoA reductase/flavanone 4-reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCGAGTCAGCGAACTCGCTGCGTGTGGC
Internal bar code:CGGATAGCTGACATCTCACTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:894
LEAP-Seq percent confirming:99.674
LEAP-Seq n confirming:14676
LEAP-Seq n nonconfirming:48
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCTTTACACGGAGCATGA
Suggested primer 2:GTACGGAAGGGGTTTGGATT