| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.138754 |
| Chromosome: | chromosome 2 |
| Location: | 1207434 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g082050 | (1 of 1) K13115 - coiled-coil domain-containing protein 130 (CCDC130) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCATGGGTGATGGGGTTCTTGTAAGCCTG |
| Internal bar code: | CGAGCTATACCGGGCTCGCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 941 |
| LEAP-Seq percent confirming: | 97.8238 |
| LEAP-Seq n confirming: | 14070 |
| LEAP-Seq n nonconfirming: | 313 |
| LEAP-Seq n unique pos: | 83 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCTCCTCGTCAGAACCAG |
| Suggested primer 2: | AGCCGACCTTCAAAGTGCTA |