| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.138757 |
| Chromosome: | chromosome 14 |
| Location: | 1157100 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g615650 | (1 of 10) 3.1.1.5 - Lysophospholipase / Phospholipase B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTTGCATGCGCCGTGTGTGGCGCCGCGC |
| Internal bar code: | TCTGTTGAGACTATTGCCGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1332 |
| LEAP-Seq percent confirming: | 99.5534 |
| LEAP-Seq n confirming: | 3344 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCACGGTACACACACTCAC |
| Suggested primer 2: | GCGTTCGGGACATACAATCT |