Insertion junction: LMJ.RY0402.138771_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.138771
Chromosome:chromosome 2
Location:557239
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g077500 TRS23 (1 of 1) PTHR23249:SF15 - TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 4; Component of TRAPP complex 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGTCGCCATGGGGGGTTGTACTGCGCCG
Internal bar code:ACGACGATAGGTTGGATAATTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:379
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ATCGTGAACCGTATTGCTCC
Suggested primer 2:ACAGGATTGGAAATTCGTGC