Insertion junction: LMJ.RY0402.138771_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.138771
Chromosome:chromosome 4
Location:2354250
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g220850 (1 of 1) 3.6.3.34//3.6.3.43//3.6.3.44 - Iron-chelate-transporting ATPase // Peptide-transporting ATPase // Xenobiotic-transporting ATPase / Steroid-transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCCCAAGTAGACCAAGGGTTGGGTTTAAA
Internal bar code:CGCAGACCGCCCCTGCGGCGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:593
LEAP-Seq percent confirming:94.4624
LEAP-Seq n confirming:16325
LEAP-Seq n nonconfirming:957
LEAP-Seq n unique pos:73

Suggested primers for confirmation by PCR

Suggested primer 1:AGGATACGAAAGGAGCAGCA
Suggested primer 2:GAACCCCACCGACTACTTCA