Insertion junction: LMJ.RY0402.138771_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.138771
Chromosome:chromosome 4
Location:2355314
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre04.g220850 (1 of 1) 3.6.3.34//3.6.3.43//3.6.3.44 - Iron-chelate-transporting ATPase // Peptide-transporting ATPase // Xenobiotic-transporting ATPase / Steroid-transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGATAGACAAGATGTCCTGTTTCGCTTCA
Internal bar code:TCGCTGTGGTCGCATACTAGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:439
LEAP-Seq percent confirming:94.8464
LEAP-Seq n confirming:9846
LEAP-Seq n nonconfirming:535
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:AACCCAGCAATTCACCAGTC
Suggested primer 2:TGCTGCTCCTTTCGTATCCT