Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.138773 |
Chromosome: | chromosome_13 |
Location: | 2550792 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g580700 | DAL2 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCGGCTGCTGCCCTTCCTGCCCTTATCCGC |
Internal bar code: | AGGGTCTGTGATCGTGTCAGCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 482 |
LEAP-Seq percent confirming: | 99.7058 |
LEAP-Seq n confirming: | 5761 |
LEAP-Seq n nonconfirming: | 17 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCCTTGAATCCCTTCTCCTC |
Suggested primer 2: | TTTGGTTTAGTTTGGGCTGG |