| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.138777 |
| Chromosome: | chromosome 12 |
| Location: | 7658157 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g554450 | 3'UTR|intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTGGTACTGAAGCAAAGATGTTGCAACC |
| Internal bar code: | CTTGTTTAGCGATCCAAAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 801 |
| LEAP-Seq percent confirming: | 98.7098 |
| LEAP-Seq n confirming: | 11782 |
| LEAP-Seq n nonconfirming: | 154 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCGCATAAAACCAAAAGG |
| Suggested primer 2: | ATGAGGCTTACGTGTTTGGG |