| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.138790 |
| Chromosome: | chromosome_2 |
| Location: | 2811937 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g094450 | PHC21 | Cell wall protein pherophorin-C21 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCCTGCAGCAAGTGCGACCCCCGTCTGGCC |
| Internal bar code: | ACATTACCCGATCGGGTAAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 753 |
| LEAP-Seq percent confirming: | 99.8296 |
| LEAP-Seq n confirming: | 4101 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTACTCTATCCGTGCCGAG |
| Suggested primer 2: | ATGCTCGCAGTGTACAGCAC |